Dip-pen nanolithography (DPN), as the name implies, is a form of nanolithography that is based conceptually on the age-old technology of dip-pen writing. The Atomic force microscope (AFM) is employed in DPN to transport molecules to a substrate for which it has affinity. In this case gold (Au) was used as the substrate because the Au surface can be very flat. Just as in dip-pen writing, the molecules, which is the ink, is transported to the AFM tip via capillary forces. The AFM is then used to form patterns which are comprised a reasonably small number of molecules in sub micron dimensions. This is analogous to transporting ink to the nib of a dip-pen and then using it to write on a piece of paper. The DPN technology has a drawback. A narrow gap capillary is formed between the AFM tip and the sample anytime an experiment is conducted in air and this condenses water from the ambient and tends to influence imaging experiments being done in the nanometer or angstrom resolution level. The molecules being transported possess the ability to anchor themselves to the substrate through a process called chemisorption, thus forming stable surface structures and hence a new type of nanolithography, DPN. DPN is also used to prepare molecular dot features to demonstrate the diffusion properties of the ink. The DPN resolution depends on certain parameters such as the grain size of the substrate, chemisorption and self-assembly which limits the diffusion of the molecules after deposition, contact time between the AFM tip and the sample surface, and thus the scan speed which affects the resolution of the DPN and relative humidity affects the resolution of the lithographic process because it controls the transportation of the ODT from the tip to the substrate.