Nanobiotechnology constitutes a new and emerging research area in biotechnological sciences. In this regard, a great effort is currently devoted to prepare nano-scale materials containing biological macromolecules, such as enzymes. These bio-functionalized nanomaterials have relevant uses in sensing and imaging technology, drug delivery systems and electronic applications. In general, the methods previously described for modifying nanostructured materials with enzymes have been based on covalent, ionic and adsorptive interactions, but no attention has been paid to the use of supramolecular chemistry for preparing mono- and multilayer enzyme nanocatalysts. A recent report describes the supramolecular-mediated immobilization of trypsin on cyclodextrin-modified gold nanospheres and the improved thermal and catalytic behaviour of this nanoimmobilized enzyme. A new strategy for preparing a catalase-superoxide dismutase bienzymatic nanocatalyst through the formation of supramolecular assemblies on the surface of gold nanospheres coated with cyclodextrins has been also described